Valenciennea sexguttata is a marine, reef-associated species that occurs in tropical climates (Baensch and Debelius 1997, H. Larson pers. comm. 2013, Moosleitner 2011). This species lives in burrows under rocks over fine, silty coral sands in lagoons and bays. It is a monogamous species that is usually found in pairs or large groups (Kuiter and Tonozuka 2001, Whiteman and Côté 2004). Maximum total length for this species is 14.0 cm (Randall et al. 1990).